an official journal of: published by:
an official journal of: published by:
Editor in Chief: RAFFAELLO COSSU


  • Stefano Caro - Department of Bioproducts and Biosystems, Aalto University, Finland
  • Matteo Ulivi - Department of Energy Engineering, Politecnico di Torino, Italy
  • Alessandro Ratto - Department of Biochemistry, Catholic University of Louvain, Belgium
  • Olli Dahl - Department of Bioproducts and Biosystems, Aalto University, Finland

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In this study, horse stable waste (horse manure, peat and wood sawdust) was processed under pyrolytic conditions. The chemical and physical properties of biochar obtained from different mixtures of horse stable residues were compared. All measurements followed an experimental design using a mixture model. This approach allowed the properties of any combination of ingredients to be predicted and the influence of each component on the final value to be estimated, with very good agreement between predicted and observed values. The results of the analysis of pH, polycyclic aromatic hydrocarbons (PAH), specific surface area (SSA) and CHNSO (carbon, hydrogen, nitrogen, sulphur and oxygen) showed that all possible combinations of materials can be used as soil amendments, since: an alkaline pH (>7) prevents soil acidification and a concentration of PAH below the limit (ΣPAH<12mg/kg) does not pose a risk to human health. A high SSA value (>180 m2/g) and a different particle size distribution (PSD) provide habitat for microorganisms, increase water retention capacity and reduce greenhouse gas (GHG) emissions from the soil.


Editorial History

  • Received: 30 May 2023
  • Revised: 10 Aug 2023
  • Accepted: 11 Sep 2023
  • Available online: 30 Sep 2023


Adánez-Rubio, I., Fonts, I., de Blas, P., Viteri, F., Gea, G., & Alzueta, M. (2021). Exploratory study of polycyclic aromatic hydrocarbons occurrence and distribution in manure pyrolysis products. Journal Of Analytical And Applied Pyrolysis, 155, 105078

ASTM. (2006). Standard Test Method for Determination of Total Solids in Biomass. W. Conshohocken, PA: US: ASTM International,

ASTM. (2013). Standard Test Method for Volatile Matter in the Analysis of Particulate Wood Fuels. W. Conshohocken, PA: US: ASTM International

ASTM. (2015). Standard Test Method for Ash in Biomass. W. Conshohocken, PA: US: ASTM International,

Bachmann, H. J., Bucheli, T. D., Dieguez-Alonso, A., Fabbri, D., Knicker, H. (2016). Toward the Standardization of Biochar Analysis: The COST Action TD1107 Interlaboratory Comparison. Journal of Agricultural and Food Chemistry, 64, 513-527

Bayan, R. (2012). Elemental Composition of Biochar from Various Biomass Feedstocks. Department of Agriculture and Environmental Sciences - Lincoln University in Missouri

Cantrell, K. B., Hunt, P. G., Uchimiya, M., Novak, J. M., Ro, K. S. (2012). Impact of pyrolysis temperature and manure source on physicochemical characteristics of biochar. Bioresource Technology , 107, 419–428

Caro, S., Dahl, O. (2021). Thermochemical valorisation of waste: Pyrolytic conversion of horse manure. Cleaner Engineering And Technology.
DOI 10.1016/j.clet.2021.100181

Eriksson, L., Johansson, E., Wikstrom, C. (1998). Mixture design—design generation, PLS analysis, and model usage. Chemiometrics and Intelligent Laboratory System, 43, 1-24

Finnish Ministry of Trade and Industry (KTM). (2000). Peat - a slowly renewable biofuel. Energia (Helsinki), 15(4-5), 43

Gai, X., Wang, H., Liu, J., Zhai, L., Liu, S., Ren, T. (2014). Effects of Feedstock and Pyrolysis Temperature on Biochar Adsorption of Ammonium and Nitrate. PLoS ONE 9(12)

García, R., Pizarro, C., Lavín, A. G., Bueno, J. L. (2013). Biomass proximate analysis using thermogravimetry. Bioresurce Technology , n. 139, 1-4

Gunarathne, V., Ashiq, A., Ramanayaka, S., Wijekoon, P., & Vithanage, M. (2019). Biochar from municipal solid waste for resource recovery and pollution remediation. Environmental Chemistry Letters, 17(3), 1225–1235

Hagberg, L., & Holmgren, K. (2008). The climate impact of future energy peat production. IVT Report B1796 IVT Swedish Environmental Research Institute Ttd. Stockholm: Sweden

Hernandez-Mena, L. E., Pécora, A. A., Beraldo, A. L. (2014). Slow Pyrolysis of Bamboo Biomass: Analysis of Biochar Properties. Chemical engineering transactions, vol. 37, 115-120

International Organization for Standardization. (2006). Soil quality — Determination of polycyclic aromatic hydrocarbons (PAH) — Gas chromatographic method with mass spectrometric detection (GC-MS) (ISO Standard No. 18287:2006).

Ioannidou, O., & Zabaniotou, A. (2007). Agricultural residues as precursors for activated carbon production—A review. Renewable and Sustainable Energy Reviews(11), 1966-2005

Jindo, K., Mizumoto, H., Sawada, Y., Sanchez-Monedero, M. A., Sonoki, T. (2014). Physical and chemical characterization of biochars derived from different agricultural residues. Biogeosciences, 11, 6613–6621

Katesa, J., & Tangsathitkulchai, C. (2013). Effect of carbonization temperature on properties of char and activated carbon from coconut shell. Suranaree J. Sci. Technol, vol. 20, n. 4, 270-278

Lee, Y., Park, J., Ryu, C., Gang, K. S., Yang, W., Park, Y.-K., Jung, J., Hyun, S. (2013). Comparison of Biochar Properties from Biomass Residues Produced by Slow Pyrolysis at 500°C. Bioresource Technology

Lehmann, J., & Joseph, S. (2009). Biochar for Environmental Management. London: UK: Earthscan

Li, Q., Lin, H., Fan, H., Zhang, S., Yuan, X., & Wang, Y. et al. (2021). Co-pyrolysis of swine manure and pinewood sawdust: Evidence of cross-interaction of the volatiles and profound impacts on product characteristics. Renewable Energy, 179, 1370-1384

Liu L, Shen G, Sun M, Cao X, Shang G, Chen P. (2014). Effect of biochar on nitrous oxide emission and its potential mechanisms. J Air Waste Manag Assoc. 64(8):894-902

Nikama, J., Keskinen, R., Närvänen, A., & Uusi-Kämppä, J. (2015). The role of bedding material in recycling the nutrients of horse manure. Equi Meeting Infrasrtuctures. Lyon: France

Official Statistics of Finland (OSF) (2021). Energy supply and consumption [e-publication]

Statistics Finland Access method: Helsinki: Finland

Pettersen C., R. (1984). The Chemical Composition of Wood. Madison, WI: US: U.S. Department of Agriculture, Forest Service, Forest Products Laboratory

Phuong, D. T., Miyanishi, T., Okayama, T., Kose, R. (2016). Pore characteristics & adsorption capacities of biochars derived from rice residues as affected by variety and pyrolysis temperature. The American Journal of Innovative Research and Applied Sciences, 179-189

Schmidt, H. P., Bucheli, T., Kammann, C., Glaser, B., Abiven, S., Leifeld, J. (2021). European Biochar Certificate - Guidelines for a sustainable production of Biochar. European Biochar Foundation (EBC)

Singh, B. P., Hatton, B. J., Singh, B., Cowie, A. L., Kathuria, A. (2010). Infleuence of Biochars on Nitrous Oxide Emission and Nitrogen Leaching from Two Contrasting Soils. In J. Environ. Qual. 39 (pp. 1224–1235)

Syifa Rinaldi, P., Nisa ‘Akromah, Z., Ramadhan, H., Husna, S., Lesmana Syamsudin, D., Bintang Panggabean, P., Agustin Murdianti, R., Haris Fatahillah, M., Perala, I., Khaula Rizqia, E., Azizah Yahya, S., Novitasari, A. and Wibowo, C. (2019). Physical and Chemical Analysis of Land in Forest Peat Swamp in Resort Pondok soar, Tanjung Putting National Park, Central Kalimantan.IOP Conference Series: Earth and Environmental Science, Volume 394, The 2nd International Conference on Tropical Silviculture: Forest Research and Innovation for Sustainable Development 10–11 September 2019, Bogor, Indonesia

US Environmental-Protection-Agency. (2017). National Water Quality Inventory. Washington, DC: US: Report to Congress EPA 841-R-16-011

Xin, X., Baoliang, C., & Lizhong, Z. (2014). Transformation, Morphology and Dissolution of Silicon and Carbon in Rice Straw-Derived Biochars under Different Pyrolytic Temperatures,. Environmental Science & Technology 48 (6), 3411-3419