ASSESSMENT OF HEAVY METALS AND PAHS IN FISH WASTE VALORISATION FOR NUTRACEUTICAL PURPOSES

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Abstract

This study presents results on contaminant quantifications for the food safety of initial biomass (fish waste) and its extract product (fish oil) resulting from the activities carried out within the VITADWASTE project. VITADWASTE is a PRIN (Progetti di Rilevante Interesse Nazionale) project funded by Italian Ministry of University Education and Research, which aims to use a multidisciplinary and sustainable approach to transform biological waste into a valuable resource, to develop a safe and effective vitamin D3 nutraceutical product for human use, based on the principles of circular economy and bioeconomy. Fish waste includes by-products such as bones, guts, heads, skin and fins, or species below the minimum conservation size and tissues not suitable for human consumption, which together account for about 20-80% of the total catches and therefore improving the management of such biomass is essential. In the VITADWASTE project, an innovative green extraction method has been developed to recover vitamin D3 from fish waste, to produce a fish oil usable for nutraceutical implementations. Here, the results of Heavy Metals (HMs) and Polycyclic Aromatic Hydrocarbons (PAHs) concentrations are reported in the fish wastes and the fish oil according to the EU regulation. The consistently low concentrations of contaminants, always within regulatory limits, confirm the safety of both the raw material and the extracted product for human use, and support the adoption of SFE as a sustainable and efficient strategy for the valorisation of marine biomass within a circular economy framework.

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Keywords

• Fish waste
• Food safety
• Fish oil
• contaminants
• Nutraceuticals
• Polycyclic aromatic hydrocarbons

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Editorial History

• Received: 29 Jul 2025
• Revised: 30 Sep 2025
• Accepted: 23 Oct 2025
• Available online: 31 Dec 2025

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References

Alessandroni, L., Boldrini, L., Leone, G.P., Frapiccini, E., Panfili M., Coisson, J.D., Segale, L., Durazzo, A., Lucarini, M., Cicero, A.F.G., Sagratini, G. Fish waste as valuable source of bioactive lipids and vitamin D3: optimization of a green extraction method by means of Supercritical Fluid Extraction (SFE). Food Chemistry (under review)

Caruso, G., Floris, R., Serangeli, C., & Di Paola, L. (2020). Fishery wastes as a yet undiscovered treasure from the sea: Biomolecules sources, extraction methods and valorization. Marine drugs, 18(12), 622.
DOI 10.3390/md18120622

Commission Regulation (EU) 2023/915 of 25 April 2023 on maximum levels for certain contaminants in food and repealing Regulation (EC) No 1881/2006 (OJ L 119, 5.5.2023, pp. 103–157)

Coppola, D., Lauritano, C., Palma Esposito, F., Riccio, G., Rizzo, C., & de Pascale, D. (2021). Fish waste: From problem to valuable resource. Marine drugs, 19(2), 116.
DOI 10.3390/md19020116

Dawson, B. R., Mantzioris, E., Connell, S. D., Nagelkerken, I., Hall, T., & Mellin, C. (2025). Fish by-products as reliable proxies to evaluate nutritional fatty acid contents in commercial fish fillets. Food Chemistry, 466, 142223.
DOI 10.1016/j.foodchem.2024.142223

De Giovanni, A., Abondio, P., Frapiccini, E., Luiselli, D., & Marini, M. (2022). Meta-analysis of a new georeferenced database on polycyclic aromatic hydrocarbons in Western and Central Mediterranean seafood. Applied Sciences, 12(6), 2776.
DOI 10.3390/ app12062776

FAO (2024) In Brief to The State of World Fisheries and Aquaculture 2024. Blue Transformation in action, FAO, Rome, Italy.
DOI 10.4060/cd0683en

Frapiccini, E., Annibaldi, A., Betti, M., Polidori, P., Truzzi, C., & Marini, M. (2018). Polycyclic aromatic hydrocarbon (PAH) accumulation in different common sole (Solea solea) tissues from the North Adriatic Sea peculiar impacted area. Marine pollution bulletin, 137, 61-68.
DOI 10.1016/j.marpolbul.2018.10.002

Frapiccini, E., Panfili, M., Guicciardi, S., Santojanni, A., Marini, M., Truzzi, C., & Annibaldi, A. (2020). Effects of biological factors and seasonality on the level of polycyclic aromatic hydrocarbons in red mullet (Mullus barbatus). Environmental Pollution, 258, 113742.
DOI 10.1016/j.envpol.2019.113742

Girolametti, F., Annibaldi, A., Illuminati, S., Damiani, E., Carloni, P., Ajdini, B., Fanelli, M., & Truzzi, C. (2024). Unlocking the elemental signature of European tea gardens: Implications for tea traceability. Food Chemistry, 453, 139641.
DOI 10.1016/j.foodchem.2024.139641

Kruijssen, F., Tedesco, I., Ward, A., Pincus, L., Love, D., & Thorne-Lyman, A. L. (2020). Loss and waste in fish value chains: A review of the evidence from low and middle-income countries. Global Food Security, 26, 100434.
DOI 10.1016/j.gfs.2020.100434

Lopes, I. G., Braos, L. B., Cruz, M. C. P., & Vidotti, R. M. (2021). Valorization of animal waste from aquaculture through composting: Nutrient recovery and nitrogen mineralization. Aquaculture, 531, 735859.
DOI 10.1016/j.aquaculture.2020.735859

Mart, L., & Nürnberg, H. W. (1986). The distribution of cadmium in the sea. In Cadmium in the Environment (pp. 28-40). Basel: Birkhäuser Basel

Meena, D. K., Mohanta, K. N., Sahoo, A. K., Singh, S. K., & Mehta, N. K. (2024). Valorizing waste to wealth: a blue revolution in environmental science. Environmental Science and Pollution Research, 31(53), 62183-62185.
DOI 10.1007/s11356-024-31938-6

Ostermeyer, U., & Schmidt, T. (2006). Vitamin D and provitamin D in fish. European Food Research and Technology, 222, 403.
DOI 10.1007/s00217-005-0086-y

Radziemska, M., Vaverková, M. D., Adamcová, D., Brtnický, M., & Mazur, Z. (2019). Valorization of fish waste compost as a fertilizer for agricultural use. Waste and Biomass Valorization, 10, 2537-2545.
DOI 10.1007/s12649-018-0288-8

Rigueto, C. V. T., de Oliveira, R., Gomes, K. S., Alessandretti, I., Nazari, M. T., Rosseto, M., ... & Dettmer, A. (2023). From waste to value‐added products: A review of opportunities for fish waste valorization. Environmental Quality Management, 33(1), 203-221.
DOI 10.1002/tqem.22040

Umesh, M.; Kumar, V.; Priyanka, K.; Kathirvel, P.; Suresh, S.; Santhosh, A.S. (2025) Fish waste valorisation through production of biodiesel and biopolymers for sustainable development: A mini review. Bioresource Technology Reports, 29, 102045.
DOI 10.1016/j.biteb.2025.102045

Zhang, Q., Liu, P., Li, S., Zhang, X., & Chen, M. (2020). Progress in the analytical research methods of polycyclic aromatic hydrocarbons (PAHs). Journal of Liquid Chromatography & Related Technologies, 43(13-14), 425-444.
DOI 10.1080/10826076.2020.1746668

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