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an official journal of: published by:
Editor in Chief: RAFFAELLO COSSU


  • Jörg Römbke - ECT Oekotoxikologie GmbH, Germany

Released under CC BY-NC-ND

Copyright: © 2018 CISA Publisher


The ecotoxicological characterization of wastes according to the European Waste List (EWL) is part of their assessment as hazardous or non-hazardous. Despite inclusion in national laws no methodological details have been fixed concerning the hazard property HP 14 (“ecotoxic”).This paper intends to discuss the classification of wastes by ecotoxicological testing, using 24 representative samples of solid wastes (identified by their EWL number) with different properties. They were sampled according to standard methods, and, with one exception (galvanic sludge), ecotoxicologically tested. No chemical investigation of the samples was performed but they were characterized according to their properties in the ABANDA data base. Nearly all of these wastes were “mirror entries” in the EWL (i.e., they can be hazardous or not depending on the concentration of hazardous substances). For the ecotoxicological characterization three aquatic tests with eluates (genotoxicity, Algae, Daphnia) as well as three terrestrial tests with solid wastes (bacteria, plants, earthworms) were conducted. All investigations were performed as limit tests with three dilution steps. Algae, plants and terrestrial bacteria were the most sensitive organisms. Since no waste eluate showed any indication of genotoxicity, the genotoxicity test should be replaced by the luminescent bacteria test (ISO 11348-3). Proposals for toxicity criteria as well as hazard classifications were taken from the literature but they were modified according to own experiences. Using these concentration limits for the classification whether these wastes are ecotoxic or not, and using different versions of the hazard classification approach, 15-19 waste samples out of 23 waste samples were classified as ecotoxic (64-83%). It is proposed to perform a plausibility check of the respective HP 14-classification. The procedure used in this contribution (i.e. sampling of the wastes, their ecotoxicological testing as well as their hazard classification) could form the basis of a standardized hazard classification approach as proposed recently in the literature. In summary, this works confirms that ecotoxicological tests are practical and sensitive in order to be used for the ecotoxicological hazard classification of very different wastes.


Editorial History

  • Received: 12 Jan 2018
  • Revised: 01 Oct 2018
  • Accepted: 31 Oct 2018
  • Available online: 03 Dec 2018


Becker, L., Scheffczyk, A., Oehlmann, J., Römbke, J. and Moser, T. (2011): Effects of boric acid on varied plants and soil organisms. J. Soils Sediment. 11: 238-248

Brackemann, H., Hagendorf, U., Hahn, J. and Vogel, U. (2000): Untersuchung von Abfällen mit biologischen Testverfahren zur Bewertung der Wassergefährdung. I. Experimentelle Ergebnisse. Z. Umweltchem. Ökotox. (UWSF) 12: 5-12

Cairns, J. (1986): The myth of the most sensitive species. BioScience 36: 670-672

CEN (Comité Européen de Normalisation) (2003): Characterisation of waste - Leaching - Compliance test for leaching of granular waste materials and sludges - Part 2: One stage batch test at a liquid to solid ratio of 10 l/kg for materials with particle size below 4 mm (without or with size reduction). CEN 12457-2. Brussels, Belgium

CEN (Comité Européen de Normalisation) (2005): Characterization of waste – Preparation of waste samples for ecotoxicity tests. CEN 14735. European Standard, Brussels, Belgium

CEN (Comité Européen de Normalisation) (2017): Construction products: Assessment of release of dangerous substances - Guidance on the use of ecotoxicity tests applied to construction products. CEN/TR 17105. Brussels, Belgium

DIBt (Deutsches Institut für Bautechnik) (2008): Merkblatt für die Bewertung der Auswirkungen von Bauprodukten auf Boden und Grundwasser. Berlin

EC (European Commission) (2000): Commission Decision of 3 May 2000 replacing Decision 94/3/EC establishing a list of wastes pursuant to Article 1(a) of Council Directive 75/442/EEC on waste and Council Decision 94/904/EC establishing a list of hazardous waste pursuant to Article 1(4) of Council Directive 91/689/EEC on hazardous waste. Official Journal of the European Communities, L 226, 6.9.2000, p 3-24

EU (European Union) (2008): Regulation on classification, labelling and packaging of substances and mixtures, amending and repealing Directives 67/548/EEC and 1999/45/EC, and amending Regulation (EC) No 1907/2006, European Parliament and Council, Art. 95 (EC). L353, 31.12.2008, pp. 1–1355

EU (European Union) (2014): Commission Regulation (EU) No 1357/2014 of 18 December 2014 replacing Annex III to Directive 2008/98/EC of the European Parliament and of the Council on waste and repealing certain Directives Text with EEA relevance

EU (European Union) (2017): Council Regulation (EU) 2017/997 of 8 June 2017 amending Annex III to Directive 2008/98/EC of the European Parliament and of the Council as regards the hazardous property HP 14 ‘Ecotoxic’)

Eurelectric (Union of the Electricity Industry) (2016): Study to assess the impacts of different approaches for hazard property “HP 14” on selected waste streams. Eurelectric comments. Report, 8 pp

Huguier, P. Manier, N., Chabot, L., Bauda, P. and Pandard, P. (2015): Ecotoxicological assessment of organic wastes spread on land: towards a proposal of a suitable test battery. Ecotox. Envir. Safe. 70: 283-293

Hund-Rinke, K. and Wiechering, H. (2001): Earthworm avoidance test for soil assessment. J. Soils Sediment. 1: 15-20

ISO (International Organization for Standardization) (2012): Water quality - Determination of the inhibition of the mobility of Daphnia magna (Cladocera, Crustacea) - Acute toxicity test. ISO 6341. Geneve, Switzerland

ISO (International Organization for Standardization) (2000): Water quality – Determination of the genotoxicity of water and waste water using the umu-test. ISO 13829. Geneve, Switzerland

ISO (International Organization for Standardization) (2002f): Soil quality — Guidance on the ecotoxicological characterization of soils and soil materials. ISO 15799. Geneva, Switzerland

ISO (International Organization for Standardization) (2004a): Water quality — Freshwater algal growth inhibition test with Desmodesmus subspicatus and Pseudokirchneriella subcapitata. ISO 8692. Geneve, Switzerland

ISO (International Organization for Standardization) (2004b): Soil quality – Determination of the Effects of Pollutants on Soil Flora. Part II: Effects of Chemicals on the Emergence and Growth of Higher Plants. ISO 11269-2. Geneve, Switzerland

ISO (International Organization for Standardization) (2007a): Water quality – Determination of the inhibitory effect on the light emission of Vibrio fischeri (Luminescent bacteria test). 11348-3. Geneva, Switzerland

ISO (International Organization for Standardization) (2008a): Soil Quality - Avoidance test for evaluating the quality of soils and the toxicity of chemicals. Test with Earthworms (Eisenia fetida/andrei). ISO 17512-1. Geneve, Switzerland

ISO (International Organization for Standardization) (2008b): Soil quality – Guidance on the assessment of tests applied in the field of ecotoxicological characterization of soils and soil materials. ISO 17616. Geneve, Switzerland

ISO (International Organization for Standardization) (2016a): Soil quality -- Contact test for solid samples using the dehydrogenase activity of Arthrobacter globiformis ISO 18187. Geneva, Switzerland

Kostka-Rick, R. (2004): Ökotoxikologische Charakterisierung von Abfällen. LfU Baden-Württemberg, Karlsruhe. Ökologische Umweltbeobachtung (Karlsruhe). 108 S

LAGA (2004): Richtlinie für das Vorgehen bei physikalischen, chemischen und biologischen Untersuchungen in Zusammenhang mit der Verwertung/Beseitigung von Abfällen. Länderarbeitsgemeinschaft Abfall (Hrsg.). Berlin, Bielefeld München: Erich Schmidt Verlag

LAGA (2012): Bund/Länder-Arbeitsgemeinschaft Abfall: LAGA-Methodensammlung Abfalluntersuchung. LAGA-Forum Abfalluntersuchung Version 2.0 Stand: 01. Oktober 2012

Marques, C.R., El-Azhari, N., Martin-Laurent, F., Pandard, P., Meline, C., Petre, A.L., Eckert, S., Zipperle, J., Váňa, M., Maly, S., Šindelářová, L., Amemori, A.S., Hofman, J., Kumar, A., Doan, H., McLaughlin, M., Richter, E. & Römbke, J. (2018): A bacterium-based contact assay for evaluating the quality of solid samples - results from an international ring test. J. Hazardous Materials 352: 139-147

Moser, H. (2008): Recommendations for the Ecotoxicological Characterization of Wastes. Federal Environmental Agency, Dessau, Germany. 25 pp

Moser, H. and Römbke, J. (2009): Ecotoxicological characterisation of waste - Results and experiences of an European ring-test. Springer Ltd., New York. 308 pp

Moser, H., Römbke, J., Donnevert, G. and Becker, R. (2011): Evaluation of biological methods for a future methodological implementation of the Hazard criterion H14 “ecotoxic” in the European waste list (2000/532/EC). Waste Manage. Res. 29: 180-187

OECD (Organisation for Economic Co-operation and Development) (1992): Fish, Acute Toxicity Test. Guideline for Testing Chemicals No. 203. Paris, France

Pandard, P., Devillers J., Charissou, A.M., Poulsen, V., Jourdain M.J., Férard, J-F., Grand, C. and Bispo, A. (2006): Selecting a Battery of Bioassays for Ecotoxicological Characterization of Wastes. Sci. Total Environ. 363: 114-125

Pandard, P. and Römbke, J. (2013): Proposal for a “Harmonized” Strategy for the Assessment of the HP 14 Property. Integ. Envir. Assess. Manag. (IEAM) 9: 665-672

Römbke, J., Moser, TH. and Moser, H. (2009): Ecotoxicological characterization of 12 incineration ashes (MWI) using 6 laboratory tests. Waste Manage. 29: 2475-2482

Römbke, J., Jänsch, S. Meier, M., Hilbeck, A., Teichmann, H. and Tappeser, B. (2010): General recommendations for soil ecotoxicological tests suitable for the Environmental Risk Assessment (ERA) of Genetically Modified Plants (GMPs). Integr. Envir. Assess. Manag. (IEAM) 6: 287-300

Römbke, J. and Ketelhut, R. (2014): Weiterentwicklung der UBA-Handlungsempfehlung zur ökotoxikologischen Charakterisierung von Abfällen. UBA-Texte 19/04, 170 pp

Wahlström, M., Laine-Ylijoki, J., Wik, O., Oberender, A. and Hjelmar, O. (2016): Hazardous waste classification. Amendments to the European Waste Classification regulation – what do they mean and what are the consequences. Report TemaNord 519, 121 pp

Weltens, R., Vanermen, R., Tirez, K., Robbens, J., Deprez, K. and Michiels, L. (2012): Screening tests for hazard classification of complex waste materials – Selection of methods. Waste Manage 32: 2208-2217

Wilke, B-M., Riepert, F., Koch, C. and Kühne, T. (2007): Ecotoxicological characterization of hazardous waste. Ecotox. Envir. Safe 70: 283-293