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PRODUCTION OF BIO-DEGRADABLE PLASTICS USING DIFFERENT WASTE RESOURCES BY STREPTOMYCES GEYSIRIENSIS SP. VITJK02

  • Jyothirmayee Kola Pratap - School of Biosciences &Technology, Vellore Institute of Technology, India
  • Krishnan Kannabiran - School of Biosciences &Technology, Vellore Institute of Technology, India

DOI 10.31025/2611-4135/2025.19517

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Copyright: © 2025 CISA Publisher

Abstract

Plastic polymers are invariably used every day for multiple applications causing huge environmental pollution globally. Biopolymers are biodegradable and considered to be a better alternative to synthetic plastics. This study explores the microbial production of Poly 3-hydroxyalkanoates (PHAs/ Poly 3-hydroxybutyrates (PHBs) using low-cost waste substrate. The polymer of hydroxyalkanoate is produced as intracellular granules, accumulates inside and serves as a source of energy for microorganisms. PHA-producing bacteria were isolated and screened using the Nile red and Sudan black B staining technique. Agro-industrial wastes like Rice bran. Sesame seed oil cake, used cooking oil, and glucose were used as carbon sources. The PHA-producing strain was characterized and identified by 16S rRNA partial genome sequencing. The extracted PHA was analyzed for PHA and PHB monomeric compounds by GC-MS analysis. PHA-producing isolate was identified as Streptomyces species and named as Streptomyces geysiriensis sp. VITJK02 by molecular taxonomic characterization. Among the carbon sources used rice bran served as a better carbon source for PHA production, and the yield was calculated to be 37.55%. Octadecanoic acid, 3-hydroxy-methyl ester, 2-butenoic acid, 1-methyl ethyl ester, 9-hexadecenoic acid, butanoic acid, 2,7-dimethyl oct-7-en-5yn-4-yl ester, butanedioic acid, dimethyl ester, pentadecanoic acid, and valeric acid, 2-tetradecyl ester were a few of the major PHA monomeric compounds observed in our study. Since PHA/PHB bioplastics are a better alternative to petroleum hydrocarbon-based synthetic plastic polymers, cheap household wastes can be used to produce PHA to minimize the cost.

Keywords

Editorial History

  • Received: 23 May 2025
  • Revised: 09 Jul 2025
  • Accepted: 29 Jul 2025
  • Available online: 09 Sep 2025

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